10.5072/example Mari, Bernard Puissegur, Marie-Pierre Barbry, Pascal Lebrigand, Kevin Institut de Pharmacologie Moleculaire et Cellulaire (IPMC), CNRS UMR6097, Universite de Nice Sophia-Antipolis, 660 route des lucioles, 06560 Valbonne - Sophia-Antipolis, France 2010 Neoplasms Transcription profiling Homo sapiens A549 DNA microarray INIST-CNRS en Experiment report http://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE18695 183 ko 3 pages PDF Creative Commons Attribution-NonCommercial-NoDerivs 3.0 Unported To identify putative novel specific targets of mir-210, we overexpressed miR-210 as well as miR-34a and a siRNA targeted against E2F3 in A549 human adenocarcinoma cells by transfecting them with synthetic pre-miRNAs or a synthetic negative pre-miRNA as control (miR-Neg). RNA samples were harvested at 48 hours post-transfection and 2 independent experiments performed in dye-swap: miR-210 versus miR-Neg ; miR-34a versus miR-Neg ; si-E2F3 versus miR-Neg ; si-control versus miR-Neg.